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HIV-1NefSMM

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Description	SMM on purified HIV-1 Nef-SF2
Motivation	to identify direct ligands for Nef protein
Biological Processes
Cellular Components
Miscellaneous Terms
Molecular Functions
Phenotypes
Therapeutic Indications
Therapeutic Uses
Screeners	Thomas Smithgall
Collaborators	Olivia McPherson John Engen
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Assay Name	Assay Description
ManualSNR(1150.0001)	Assay Vol(3mL) biological object(Protein: HIV-1_Nef-his) Exp time(30m) buffer(Solution: Tris-NaCl-DTT) detection reagent(Protein: aHIS-A647) Detect rgt incubate time(30m at 25C) Detector(Axon4000B) Detector method(SMM) LambdaEx(647) LambdaEm(635)
ManualSNR(1150.0002)	Assay Vol(3mL) biological object(Protein: HIV-1_Nef-his) Exp time(30m) buffer(Solution: Tris-NaCl-DTT) detection reagent(Protein: aHIS-A647) Detect rgt incubate time(30m at 25C) Detector(Axon4000B) Detector method(SMM) LambdaEx(647) LambdaEm(635)

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